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ASCP ASCP-MLT Exam Syllabus Topics:

SectionObjectives
Laboratory Operations- Quality and safety in the clinical lab
  • 1. Quality control and assurance
    • 2. Laboratory safety and regulations
      Microbiology- Clinical microbiological techniques
      • 1. Culture and sensitivity testing
        • 2. Bacteriology and organism identification
          Hematology- Blood cell analysis and disorders
          • 1. CBC and differential interpretation
            • 2. Anemias and leukemias overview
              Clinical Chemistry- Metabolic and analytical testing
              • 1. Endocrine and metabolic panels
                • 2. Enzymes and electrolytes
                  Blood Banking / Immunohematology- Blood group systems and antibody identification
                  • 1. ABO and Rh systems
                    • 2. Crossmatching procedures
                      Urinalysis and Body Fluids- Fluid analysis and interpretation
                      • 1. Urine chemical and microscopic examination
                        • 2. CSF and other body fluids

                          ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

                          1. Chain-of-Custody procedures must be followed for

                          A) blood specimens for alcohol level determination
                          B) Throat swabs of groups A beta streptococcus screening
                          C) Therapeutic drug threshold determinations
                          D) routine urinalysis for glucose and ketones


                          2. The laboratory uses flammable chemicals for processing and staining patient specimens. One type of chemical used is ethanol. What is the proper way to store this chemical in the laboratory?

                          A) In a flame-resistant cabinet
                          B) On the shelf with other hazardous chemicals
                          C) Under the sink in the laboratory
                          D) In the back of the fume hood


                          3. Blood collected in CPDA-1 may be stored at 1 - 6 oC for up to 35 days.
                          How long may blood be stored using CPDA-1 preservative prior to transfusion?

                          A) 5 days
                          B) 15 days
                          C) 35 days
                          D) 25 days


                          4. Hemolytic transfusion reactions seldom occur because the incidence of unexpected antibodies in random patients is relatively low, e.g., 3-5% is sometimes cited.
                          Being transfused with O Rh negative RBC is irrelvant if you have an unexpected antibody like anti-K or anti-c.
                          Incompatible red cells may "bleed out" but only if bleeding is brisk. Even then, a hemolytic reaction may occur later once the patient's antibody rebounds and destroys remaining antigen-positive donor red cells.
                          It's true that some patients have only cold-reactive antibodies that will not react at body temperature. But this does not explain why warm-reactive red cell antibodies cause few reactions Why do so few patients transfused with un-crossmatched red cells in an emergency experience a hemolytic transfusion reaction? Select the one best reason.

                          A) They usually receive group O Rh negative red cells; a hemolytic transfusion reaction will never occur if O Rh-negative red cells are transfused.
                          B) They hemorrhage so severely that incompatible donor red cells "bleed out" before a reaction occurs.
                          C) Some patients have cold-reactive antibodies that will not react at body temperature.
                          D) The incidence of unexpected red cell antibodies is relatively low.


                          5. Platelets do not circulate in inactivated, spiny forms. The spiny, sticky form of the platelet is initiated once the platelets become activated in response to blood vessel damage.
                          Which of the following is not true in terms of platelet characteristics?

                          A) Possess an inherent sticky property that aids in adhesion and aggregation.
                          B) Produced in the bone marrow by megakaryocytes.
                          C) Are normally found circulating in their inactive, spiny form.
                          D) Have a lifespan of roughly 9-12 days.


                          Solutions:

                          Question # 1
                          Answer: A
                          Question # 2
                          Answer: A
                          Question # 3
                          Answer: C
                          Question # 4
                          Answer: D
                          Question # 5
                          Answer: A

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